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pro-q diamond phosphoprotein gel staining kit  (Thermo Fisher)


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    Thermo Fisher pro-q diamond phosphoprotein gel staining kit
    Pro Q Diamond Phosphoprotein Gel Staining Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pro-q+diamond+phosphoprotein+gel+staining+kit/pro+q+diamond+phosphoprotein+gel+stain/pmc12012799-275-37-43
    Average 90 stars, based on 1 article reviews
    pro-q diamond phosphoprotein gel staining kit - by Bioz Stars, 2026-08
    90/100 stars

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    pro-q diamond phosphoprotein gel staining kit  (Thermo Fisher)
    90
    Thermo Fisher pro-q diamond phosphoprotein gel staining kit
    Pro Q Diamond Phosphoprotein Gel Staining Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pro-q+diamond+phosphoprotein+gel+staining+kit/pro+q+diamond+phosphoprotein+gel+stain/pmc12012799-275-37-43
    Average 90 stars, based on 1 article reviews
    pro-q diamond phosphoprotein gel staining kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    pro-q diamond phosphoprotein gel stain kit  (Thermo Fisher)
    90
    Thermo Fisher pro-q diamond phosphoprotein gel stain kit
    (A) MARK3-mediated Ser-937/Ser-978 phosphorylation of SSH1 promotes its binding to 14-3-3. HA-MARK3 was expressed in HEK293T cells, purified using anti-HA immunoprecipitation, and subjected to in vitro kinase reaction using recombinant [Myc+(His) 6 ]-SSH1(WT or 2SA) as a substrate. After centrifugation, supernatants were subjected to in vitro pull-down assay with GST-14-3-3γ bound to glutathione-Sepharose, and the precipitates were analyzed by immunoblotting with anti-Myc and anti-pS-978 antibodies. GST-14-3-3γ was analyzed by CBB staining. The initial supernatants were directly subjected to SDS-PAGE and immunoblotted with anti-Myc and anti-pS-978 antibodies. (B) MARK3 knockdown reduces the interaction between SSH1 and 14-3-3 proteins in cells. HEK293T cells were transfected with the control or MARK3-targeting shRNAs. Endogenous MARK3 expression was analyzed by immunoblotting using an anti-MARK3 antibody. Cell lysates were immunoprecipitated with an anti-SSH1 antibody and the immunoprecipitates were analyzed by immunoblotting with anti-SSH1 and anti-14-3-3 antibodies. Quantitative data are shown as the means ± S.D. of three independent experiments. *, p<0.01. (C) MARK3-mediated Ser-937/Ser-978 phosphorylation and subsequent 14-3-3 binding suppress <t>the</t> <t>cofilin-phosphatase</t> activity of SSH1. [Myc+(His) 6 ]-SSH1(WT or 2SA) was phosphorylated by HA-MARK3 as shown in (B). After centrifugation, the supernatants were incubated with indicated amount of GST-14-3-3γ, and then subjected to in vitro cofilin-phosphatase assay, using P-cofilin as a substrate, in the presence of F-actin. P-cofilin and total cofilin were detected using <t>Pro-Q</t> and CBB staining, respectively.
    Pro Q Diamond Phosphoprotein Gel Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pro-q+diamond+phosphoprotein+gel+staining+kit/bio_rxiv__2023__10__15__562441-169-7-13
    Average 90 stars, based on 1 article reviews
    pro-q diamond phosphoprotein gel stain kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    pro-q® diamond phosphoprotein gel stain kit  (Thermo Fisher)
    90
    Thermo Fisher pro-q® diamond phosphoprotein gel stain kit
    (A) MARK3-mediated Ser-937/Ser-978 phosphorylation of SSH1 promotes its binding to 14-3-3. HA-MARK3 was expressed in HEK293T cells, purified using anti-HA immunoprecipitation, and subjected to in vitro kinase reaction using recombinant [Myc+(His) 6 ]-SSH1(WT or 2SA) as a substrate. After centrifugation, supernatants were subjected to in vitro pull-down assay with GST-14-3-3γ bound to glutathione-Sepharose, and the precipitates were analyzed by immunoblotting with anti-Myc and anti-pS-978 antibodies. GST-14-3-3γ was analyzed by CBB staining. The initial supernatants were directly subjected to SDS-PAGE and immunoblotted with anti-Myc and anti-pS-978 antibodies. (B) MARK3 knockdown reduces the interaction between SSH1 and 14-3-3 proteins in cells. HEK293T cells were transfected with the control or MARK3-targeting shRNAs. Endogenous MARK3 expression was analyzed by immunoblotting using an anti-MARK3 antibody. Cell lysates were immunoprecipitated with an anti-SSH1 antibody and the immunoprecipitates were analyzed by immunoblotting with anti-SSH1 and anti-14-3-3 antibodies. Quantitative data are shown as the means ± S.D. of three independent experiments. *, p<0.01. (C) MARK3-mediated Ser-937/Ser-978 phosphorylation and subsequent 14-3-3 binding suppress <t>the</t> <t>cofilin-phosphatase</t> activity of SSH1. [Myc+(His) 6 ]-SSH1(WT or 2SA) was phosphorylated by HA-MARK3 as shown in (B). After centrifugation, the supernatants were incubated with indicated amount of GST-14-3-3γ, and then subjected to in vitro cofilin-phosphatase assay, using P-cofilin as a substrate, in the presence of F-actin. P-cofilin and total cofilin were detected using <t>Pro-Q</t> and CBB staining, respectively.
    Pro Q® Diamond Phosphoprotein Gel Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pro-q+diamond+phosphoprotein+gel+staining+kit/pro+q+diamond+phosphoprotein+gel+stain/pm32694166-351-11-17
    Average 90 stars, based on 1 article reviews
    pro-q® diamond phosphoprotein gel stain kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    pro q diamond phosphoprotein gel stain kit  (Thermo Fisher)
    86
    Thermo Fisher pro q diamond phosphoprotein gel stain kit
    (A) MARK3-mediated Ser-937/Ser-978 phosphorylation of SSH1 promotes its binding to 14-3-3. HA-MARK3 was expressed in HEK293T cells, purified using anti-HA immunoprecipitation, and subjected to in vitro kinase reaction using recombinant [Myc+(His) 6 ]-SSH1(WT or 2SA) as a substrate. After centrifugation, supernatants were subjected to in vitro pull-down assay with GST-14-3-3γ bound to glutathione-Sepharose, and the precipitates were analyzed by immunoblotting with anti-Myc and anti-pS-978 antibodies. GST-14-3-3γ was analyzed by CBB staining. The initial supernatants were directly subjected to SDS-PAGE and immunoblotted with anti-Myc and anti-pS-978 antibodies. (B) MARK3 knockdown reduces the interaction between SSH1 and 14-3-3 proteins in cells. HEK293T cells were transfected with the control or MARK3-targeting shRNAs. Endogenous MARK3 expression was analyzed by immunoblotting using an anti-MARK3 antibody. Cell lysates were immunoprecipitated with an anti-SSH1 antibody and the immunoprecipitates were analyzed by immunoblotting with anti-SSH1 and anti-14-3-3 antibodies. Quantitative data are shown as the means ± S.D. of three independent experiments. *, p<0.01. (C) MARK3-mediated Ser-937/Ser-978 phosphorylation and subsequent 14-3-3 binding suppress <t>the</t> <t>cofilin-phosphatase</t> activity of SSH1. [Myc+(His) 6 ]-SSH1(WT or 2SA) was phosphorylated by HA-MARK3 as shown in (B). After centrifugation, the supernatants were incubated with indicated amount of GST-14-3-3γ, and then subjected to in vitro cofilin-phosphatase assay, using P-cofilin as a substrate, in the presence of F-actin. P-cofilin and total cofilin were detected using <t>Pro-Q</t> and CBB staining, respectively.
    Pro Q Diamond Phosphoprotein Gel Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pro-q+diamond+phosphoprotein+gel+staining+kit/pmc06744581-84-10-16
    Average 86 stars, based on 1 article reviews
    pro q diamond phosphoprotein gel stain kit - by Bioz Stars, 2026-08
    86/100 stars
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    (A) MARK3-mediated Ser-937/Ser-978 phosphorylation of SSH1 promotes its binding to 14-3-3. HA-MARK3 was expressed in HEK293T cells, purified using anti-HA immunoprecipitation, and subjected to in vitro kinase reaction using recombinant [Myc+(His) 6 ]-SSH1(WT or 2SA) as a substrate. After centrifugation, supernatants were subjected to in vitro pull-down assay with GST-14-3-3γ bound to glutathione-Sepharose, and the precipitates were analyzed by immunoblotting with anti-Myc and anti-pS-978 antibodies. GST-14-3-3γ was analyzed by CBB staining. The initial supernatants were directly subjected to SDS-PAGE and immunoblotted with anti-Myc and anti-pS-978 antibodies. (B) MARK3 knockdown reduces the interaction between SSH1 and 14-3-3 proteins in cells. HEK293T cells were transfected with the control or MARK3-targeting shRNAs. Endogenous MARK3 expression was analyzed by immunoblotting using an anti-MARK3 antibody. Cell lysates were immunoprecipitated with an anti-SSH1 antibody and the immunoprecipitates were analyzed by immunoblotting with anti-SSH1 and anti-14-3-3 antibodies. Quantitative data are shown as the means ± S.D. of three independent experiments. *, p<0.01. (C) MARK3-mediated Ser-937/Ser-978 phosphorylation and subsequent 14-3-3 binding suppress the cofilin-phosphatase activity of SSH1. [Myc+(His) 6 ]-SSH1(WT or 2SA) was phosphorylated by HA-MARK3 as shown in (B). After centrifugation, the supernatants were incubated with indicated amount of GST-14-3-3γ, and then subjected to in vitro cofilin-phosphatase assay, using P-cofilin as a substrate, in the presence of F-actin. P-cofilin and total cofilin were detected using Pro-Q and CBB staining, respectively.

    Journal: bioRxiv

    Article Title: MARK3-mediated Slingshot-1 phosphorylation is essential for polarized lamellipodium formation

    doi: 10.1101/2023.10.15.562441

    Figure Lengend Snippet: (A) MARK3-mediated Ser-937/Ser-978 phosphorylation of SSH1 promotes its binding to 14-3-3. HA-MARK3 was expressed in HEK293T cells, purified using anti-HA immunoprecipitation, and subjected to in vitro kinase reaction using recombinant [Myc+(His) 6 ]-SSH1(WT or 2SA) as a substrate. After centrifugation, supernatants were subjected to in vitro pull-down assay with GST-14-3-3γ bound to glutathione-Sepharose, and the precipitates were analyzed by immunoblotting with anti-Myc and anti-pS-978 antibodies. GST-14-3-3γ was analyzed by CBB staining. The initial supernatants were directly subjected to SDS-PAGE and immunoblotted with anti-Myc and anti-pS-978 antibodies. (B) MARK3 knockdown reduces the interaction between SSH1 and 14-3-3 proteins in cells. HEK293T cells were transfected with the control or MARK3-targeting shRNAs. Endogenous MARK3 expression was analyzed by immunoblotting using an anti-MARK3 antibody. Cell lysates were immunoprecipitated with an anti-SSH1 antibody and the immunoprecipitates were analyzed by immunoblotting with anti-SSH1 and anti-14-3-3 antibodies. Quantitative data are shown as the means ± S.D. of three independent experiments. *, p<0.01. (C) MARK3-mediated Ser-937/Ser-978 phosphorylation and subsequent 14-3-3 binding suppress the cofilin-phosphatase activity of SSH1. [Myc+(His) 6 ]-SSH1(WT or 2SA) was phosphorylated by HA-MARK3 as shown in (B). After centrifugation, the supernatants were incubated with indicated amount of GST-14-3-3γ, and then subjected to in vitro cofilin-phosphatase assay, using P-cofilin as a substrate, in the presence of F-actin. P-cofilin and total cofilin were detected using Pro-Q and CBB staining, respectively.

    Article Snippet: P-cofilin and total cofilin were analyzed by Pro-Q Diamond phosphoprotein gel stain kit (Invitrogen) and Coomassie brilliant blue (CBB) staining, respectively.

    Techniques: Binding Assay, Purification, Immunoprecipitation, In Vitro, Recombinant, Centrifugation, Pull Down Assay, Western Blot, Staining, SDS Page, Transfection, Expressing, Activity Assay, Incubation, Phosphatase Assay